Antagonistic activity expressed by Shigella sonnei: identification of a putative new bacteriocin
AUTOR(ES)
Sousa, Mireille Angela Bernardes, Farias, Luiz de Macedo, Oliveira, Patricia Luciana de, Moreira, Jaqueline Silvana, Apolonio, Ana Carolina Morais, Oliveira, Jamil Silvano, Santoro, Marcelo Matos, Mendes, Edilberto Nogueira, Magalhaes, Paula Prazeres
FONTE
Mem. Inst. Oswaldo Cruz
DATA DE PUBLICAÇÃO
2013-09
RESUMO
Bacteriocins are antibacterial, proteinaceous substances that mediate microbial dynamics. Bacteriocin production is a highly disseminated property among all major lineages of bacteria, including Shigella. In this paper, we addressed the purification and characterisation of a bacteriocin produced by a Shigella sonnei strain (SS9) isolated from a child with acute diarrhoea. The substance was purified through ammonium-sulphate precipitation and sequential steps of chromatography. The intracellular fraction obtained at 75% ammonium sulphate maintained activity following exposure to pH values from 1-11 and storage at -80ºC for more than two years and was inactivated by high temperatures and proteases. The molecular mass of the purified bacteriocin was determined by mass spectrometry to be 18.56 kDa. The N-terminal sequence of the bacteriocin did not match any other antibacterial proteins described. A putative new bacteriocin produced by S. sonnei has been detected. This bacteriocin may represent a newly described protein or a previously described protein with a newly detected function. Considering that SS9 expresses antagonism against other diarrhoeagenic bacteria, the bacteriocin may contribute to S. sonnei virulence and is potentially applicable to either preventing or controlling diarrhoeal disease.
Documentos Relacionados
- Purification and the Ultrastructure of a Bacteriocin Produced from Shigella sonnei Strain 100052
- Beta-Lactamase Activity in Shigella sonnei
- SALICIN FERMENTATION BY SHIGELLA SONNEI
- Genetic analysis of an invasion region by use of a Tn3-lac transposon and identification of a second positive regulator gene, invE, for cell invasion of Shigella sonnei: significant homology of invE with ParB of plasmid P1.
- Bacteriophage typing of Shigella sonnei.