Antigenic Specificity of Neutralizing Antibody to Cholera Toxin
AUTOR(ES)
Peterson, Johnny W.
RESUMO
Selected rabbit antisera to cholera toxin antigens and convalescent cholera patient sera were analyzed using the permeability factor neutralization test and two sensitive in vitro serological assays specific for cholera toxin, cholera toxin A subunit, and cholera toxin B subunit. The results indicated that antisera to cholera toxin contained toxin-neutralizing activity as well as antibodies specific for both the A subunit and B subunit. It was clearly established that antisera to B subunit, devoid of significant anti-A subunit activity, neutralized the vascular permeability activity of cholera toxin. Antisera to A subunit contained neutralizing antibodies and antibodies to both A and B subunits. Absorption with B subunit removed both the toxin-neutralizing and anti-B subunit activities, while the anti-A activity was unaffected. Neutralizing antibody titers of rabbits immunized with B subunit were also observed to be significantly higher than neutralizing antibody titers of sera from A subunit-immunized rabbits, despite the overall similarity in anti-B subunit titers as determined by passive hemagglutination and radioimmunoassay of sera from the two groups of rabbits. Anti-α chain sera neither neutralized cholera toxin nor possessed significant antitoxin or anti-B subunit titers as determined by passive hemagglutination and radioimmunoassay. The anti-α chain sera contained high levels of antibody specific for A subunit, which is consistent with the hypothesis that the α chain is part of the A subunit structure. In contrast, the γ chain was not shown to be antigenic. Sera from convalescent cholera patients possessed toxin-neutralizing antibody as well as passive hemagglutination and radioimmunoassay antibody against both A and B subunits.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=414373Documentos Relacionados
- Immunity to Cholera: Relation of Fraction II of Type 2 Cholera Toxin to Vibriocidal Antibody
- Characterization of the Antigenic Determinants of Cholera Toxin Subunits
- Toxin-neutralizing effect of antibody against subtilisin-digested tetanus toxin.
- Suppression of the intestinal immune response to cholera toxin by specific serum antibody.
- Radioimmunoassay for the Antigenic Determinants of Cholera Toxin and Its Components