Application of Real-Time Fluorescent PCR for Quantitative Assessment of Neospora caninum Infections in Organotypic Slice Cultures of Rat Central Nervous System Tissue
AUTOR(ES)
Müller, Norbert
FONTE
American Society for Microbiology
RESUMO
The previously described Nc5-specific PCR test for the diagnosis of Neospora caninum infections was used to develop a quantitative PCR assay which allows the determination of infection intensities within different experimental and diagnostic sample groups. The quantitative PCR was performed by using a dual fluorescent hybridization probe system and the LightCycler Instrument for online detection of amplified DNA. This assay was successfully applied for demonstrating the parasite proliferation kinetics in organotypic slice cultures of rat brain which were infected in vitro with N. caninum tachyzoites. This PCR-based method of parasite quantitation with organotypic brain tissue samples can be regarded as a novel ex vivo approach for exploring different aspects of cerebral N. caninum infection.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=120097Documentos Relacionados
- Quantitative Detection of Neospora caninum in Bovine Aborted Fetuses and Experimentally Infected Mice by Real-Time PCR
- Real-Time Quantitative PCR for Assessment of Abundance of Pseudoalteromonas Species in Marine Samples
- Real-Time Quantitative PCR for Detection of Helicobacter pylori
- Real-Time PCR for Quantitative Detection of Toxoplasma gondii
- Real-Time Quantitative PCR for Human Herpesvirus 6 DNA
