Assay for Helminthosporium maydis Toxin-binding Activity in Plants 1

AUTOR(ES)

Ireland, Carrie R.

RESUMO

A relatively rapid and sensitive assay is described for assessing the binding of Helminthosporium maydis Race T14 C-toxins I and II to plant components. The technique is a modification of the one of Haddad and Birge (J. Biol. Chem. 250: 299-303, 1975), and utilizes dextran-coated charcoal as an adsorbent for the unreacted toxin and employs a Millipore filter to isolate the protein-toxin complex.

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