Assay of Typhoid Vaccines with Salmonella typhosa-Salmonella typhimurium Hybrids
AUTOR(ES)
Diena, B. B.
RESUMO
Salmonella typhimurium hybrids expressing the S. typhosa antigens 9, d, and Vi were constructed by genetic crosses with an S. typhosa Hfr donor. The hybrids retained the same degree of mouse virulence as their S. typhimurium parent strain, the minimum lethal dose being less than 50 organisms when tested either in C57 black mice or Swiss white mice. Vaccination of the Swiss white mice with S. typhosa Ty2 vaccines prepared by acetone treatment, alcohol treatment, or heat-killing conferred significant protection against challenge by the hybrid strains but not against their S. typhimurium parent. Both the acetone-treated and alcohol-treated typhoid vaccines were markedly more protective than the heat-killed, phenol-preserved vaccine.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=422630Documentos Relacionados
- Ineffectiveness of Vi and chemically treated endotoxins as typhoid vaccines in mice challenged with a Salmonella typhosa-Salmonella typhimurium hybrid.
- Evaluation of Two Salmonella typhimurium Hybrids as Challenge Organisms in a System for the Assay of Typhoid Vaccines
- Lytic Replication of Coliphage Lambda in Salmonella typhosa Hybrids
- VI-NEGATIVE STRAINS OF SALMONELLA TYPHOSA: ATTEMPTS TO INDUCE W-V REVERSION AND THE USE OF NON-VI STRAINS IN EVALUATING TYPHOID VACCINES
- Transduction of SALMONELLA TYPHIMURIUM-SALMONELLA MONTEVIDEO Hybrids