Assembly of an active chromatin structure during replication.
AUTOR(ES)
Weintraub, H
RESUMO
MSB cells were pulse labeled with 3H-thymidine and the isolated nuclei digested with either staphylococcal nuclease (to about 40% acid solubility) or DNase I (to 15% acid solubility). The purified, nuclease resistant single-copy DNA was then hybridized to nuclear RNA (nRNA). The results of these experiments show that actively transcribed genes are assembled into nucleosome-like structures within 5-10 nucleosomes of the replication fork and that they also acquire a conformation characteristic of actively transcribed nucleosomes (ie, a DNase I sensitive structure) within 20 nucleosomes of the fork. Assuming DNA sequence specific interactions are required for establishing a DNase I sensitive conformation on active genes during each round of replication, our results indicate that a specific recognition event can occur very rapidly and very specifically in eukaryotic cells. The results are discussed in terms of the possible mechanisms responsible for propagating active, chromosomal conformations from mother cells to daughter cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=328055Documentos Relacionados
- Chromatin structure: a property of the higher structures of chromatin and in the time course of its formation during chromatin replication.
- Stepwise assembly of chromatin during DNA replication in vitro.
- Effects of cell cycle dependent histone H1 phosphorylation on chromatin structure and chromatin replication.
- Topoisomerase function during replication-independent chromatin assembly in yeast.
- Assembly of transfected DNA into chromatin: structural changes in the origin-promoter-enhancer region upon replication.
