Assembly of chick brain tubulin onto flagellar microtubules from Chlamydomonas and sea urchin sperm.
AUTOR(ES)
Binder, L I
RESUMO
Flagellar microtubules from Chlamydomonas and sea urchin sperm were used as in vitro assembly sites for chick brain tubulin. Brain microtubules assembled onto the A-tubules, central tubules, and, to a limited extent, onto the distal ends of the axonemes at low tubulin concentrations and onto distal and proximal ends at high tubulin concentrations; however, the rate of assembly onto the distal end was always greater. The rate of neurotubule assembly onto axonemes was shown to be dependent upon tubulin concentration and a forward rate constant for assembly was determined.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=432478Documentos Relacionados
- Isolation and Partial Characterization of α- and β-Tubulin from Outer Doublets of Sea-Urchin Sperm and Microtubules of Chick-Embryo Brain
- Membrane potential depolarization and increased intracellular pH accompany the acrosome reaction of sea urchin sperm.
- Calcium channel antagonists inhibit the acrosome reaction and bind to plasma membranes of sea urchin sperm.
- The phosphocreatine shuttle of sea urchin sperm: flagellar creatine kinase resulted from a gene triplication.
- A stable alpha-helical element in the carboxy-terminal domain of free and chromatin-bound histone H1 from sea urchin sperm.