Assembly of RNA polymerase II preinitiation complexes before assembly of nucleosomes allows efficient initiation of transcription on nucleosomal templates.
AUTOR(ES)
Knezetic, J A
RESUMO
We have previously shown that assembly of nucleosomes on the DNA template blocks transcription initiation by RNA polymerase II in vitro. In the studies reported here, we demonstrate that assembly of a complete RNA polymerase II preinitiation complex before nucleosome assembly results in nucleosomal templates which support initiation in vitro as efficiently as naked DNA. Control experiments prove that our observations are not the result of slow displacement of nucleosomes by the transcription machinery during chromatin assembly, nor are they an artifact of inefficient nucleosome deposition on templates already bearing an RNA polymerase. Thus, the RNA polymerase II preinitiation complex appears to be resistant to disruption by subsequent nucleosome assembly.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=363538Documentos Relacionados
- Initiation of transcription on nucleosomal templates.
- In vitro initiation of transcription by RNA polymerase II on in vivo-assembled chromatin templates.
- Isolation of stable preinitiation, initiation, and elongation complexes from RNA polymerase II-directed transcription.
- Multiple rounds of transcription by RNA polymerase II at covalently cross-linked templates.
- N4 virion DNA dependent-RNA polymerase: initiation sequences utilized by the enzyme on heterologous templates.
