Assembly of viral membranes. I. Association of vesicular stomatitis virus membrane proteins and membranes in a cell-free system.
AUTOR(ES)
Morrison, T G
RESUMO
We report here an in vitro system designed to study the interactions of vesicular stomatitis virus (VSV) proteins with cellular membranes. We have synthesized the VSV nucleocapsid (N) protein, nonstructural (NS) protein, glycoprotein (G protein), and membrane (M) protein in a wheat germ, cell-free, protein-synthesizing system directed by VSV 12 to 18S RNA. When incubated at low salt concentrations with purified cytoplasmic membranes derived from Chinese hamster ovary cells, the VSV M andG proteins bind to membranes, whereas the VSV N and NS proteins do not. The VSV M protein binds to membranes in low or high divalent cation concentrations, whereas binding of significant amounts of G protein requires at least 5 mM magnesium acetate concentrations.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=353844Documentos Relacionados
- Initiation and replication of vesicular stomatitis virus genome RNA in a cell-free system.
- Cell-free synthesis and assembly of vesicular stomatitis virus nucleocapsids.
- Assembly of viral membranes: nature of the association of vesicular stomatitis virus proteins to membranes.
- Transport of vesicular stomatitis virus glycoprotein in a cell-free extract.
- Human immunodeficiency virus integration in a cell-free system.
