Assignment of the human dihydrofolate reductase gene to the q11----q22 region of chromosome 5.

AUTOR(ES)

Funanage, V L

RESUMO

Cells from a dihydrofolate reductase-deficient Chinese hamster ovary cell line were hybridized to human fetal skin fibroblast cells. Nineteen dihydrofolate reductase-positive hybrid clones were isolated and characterized. Cytogenetic and biochemical analyses of these clones have shown that the human dihydrofolate reductase (DHFR) gene is located on chromosome 5. Three of these hybrid cell lines contained different terminal deletions of chromosome 5. An analysis of the breakpoints of these deletions has demonstrated that the DHFR gene resides in the q11----q22 region.

Documentos Relacionados

• Assignment of the human gene for the glucocorticoid receptor to chromosome 5.
• Argininosuccinic aciduria: assignment of the argininosuccinate lyase gene to the pter to q22 region of human chromosome 7 by bioautography.
• Four-base-pair deletion polymorphism at D5S71 (C11p11) linked to APC in the human chromosome 5q21-q22 region.
• Regional gene assignment of human porphobilinogen deaminase and esterase A4 to chromosome 11q23 leads to 11qter.
• Molecular cloning of the c-fms locus and its assignment to human chromosome 5.