Auxin from the Developing Inflorescence Is Required for the Biosynthesis of Active Gibberellins in Barley Stems1

AUTOR(ES)

Wolbang, Carla M.

FONTE

The American Society for Plant Biologists

RESUMO

Multiple gibberellins (GAs) were quantified in the stems of intact, decapitated, and decapitated auxin-treated barley (Hordeum vulgare) plants. Removal of the developing inflorescence reduced the endogenous levels of indole-3-acetic acid (IAA), GA1, and GA3 and increased the level of GA29 in internodal and nodal tissues below the site of excision. Application of IAA to the excised stump restored GA levels to normal in almost all cases. The conversion of [14C]GA20 to bioactive [14C]GA1 and of [14C]GA5 to bioactive [14C]GA3 was reduced by decapitation, and IAA application was able to restore conversion rates back to the levels found in intact plants. The amount of mRNA for the principal vegetative 3-oxidase (converting GA20 to GA1, and GA5 to GA3) was decreased in decapitated plants and restored by IAA application. The results indicate that the inflorescence of barley is a source of IAA that is transported basipetally into the internodes and nodes where bioactive GA1 and GA3 are biosynthesized. Thus, IAA is required for normal GA biosynthesis in stems, acting at multiple steps in the latter part of the pathway.

Documentos Relacionados

• Methyl Jasmonate Induces Traumatic Resin Ducts, Terpenoid Resin Biosynthesis, and Terpenoid Accumulation in Developing Xylem of Norway Spruce Stems1
• Analysis of Native Gibberellins in the Internode, Nodes, Leaves, and Inflorescence of Developing Avena Plants 1
• Metabolism of Uridine 5′-Diphosphate-Glucose in Golgi Vesicles from Pea Stems1
• Spatiotemporal Profiling of Starch Biosynthesis and Degradation in the Developing Barley Grain1[W]
• Auxin Is Required for Leaf Vein Pattern in Arabidopsis1