Avaliação histológica e imunolocalização de STRO-1 e BMP-4 em molares de ratos com polpa vital ou necrose pulpar induzida durante o desenvolvimento radicular
AUTOR(ES)
Daiana Elisabeth Böttcher
FONTE
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia
DATA DE PUBLICAÇÃO
2012
RESUMO
Objective: the aim of this study was to characterize the features of live and necrotic dental pulps of molar teeth in rats with developing roots, and to assess the expression of STRO-1 and BMP-4. Methods: twenty-four 4-month-old male Wistar rats were used. To induce necrosis, the pulps of the first lower left molars were exposed to the oral environment. The lower right molars served as the control non-operated group. Euthanasia of animals was carried out at 7, 10, 13 and 16 weeks and the jaws were dissected and processed for histological analysis by hematoxylin-eosin and immunohistochemistry. The periapical inflammatory reaction, the characteristics of the pulp tissue (inflammatory cells, vascularity, odontoblasts, degeneration, tissue destruction), closure of the foramen and presence of resorptions were described at each period. Results: all teeth from the test group presented pulp necrosis, occurring discontinuation of root development. Meanwhile, the teeth of the control group showed normal development of dental tissues. BMP-4-positive cells were detected in the pulp of teeth from the control group. However, there was no expression in teeth of the test group. For STRO-1, expression was more evident in the blood vessels, both for the control group and for the test group. Apical papilla evidenced no BMP-4 nor STRO-1 expression. Conclusion: Immunohistochemistry was not sufficient to evaluate the presence of stem cells in the apical papilla region of nonvital immature teeth. It is possible that the inflammatory process has blocked the expression of STRO-1 and BMP-4., Probably, infection has negatively modulated this expression.
ASSUNTO(S)
endodontics endodontia dental pulp polpa dentaria immature necrotic teeth células-tronco mesenchymal stem cells
ACESSO AO ARTIGO
http://hdl.handle.net/10183/52700Documentos Relacionados
- Investigação de polimorfismos na região promotora do gene BMP-4 em individuos com agenesia dental
- Metamorfose cálcica da polpa e necrose pulpar asséptica no planejamento ortodôntico
- Uso do "curativo de demora" em diferentes tempos no tratamento endodôntico de cães com necrose pulpar induzida
- Estudo da associação entre o gene BMP-4 e anomalias congênitas do rim e trato urinário
- Involvement of Ras/Raf/AP-1 in BMP-4 signaling during Xenopus embryonic development.