Avaliation of the blood lymphocytes kynetics in bovines after simultaneous application with the synthetics immunogenics anti-Babesia bovis (Sbbo23290) and anti-Ripicephalus (Boophilus) microplus (Bm7462) / Avaliação da cinética leucocitária sangüínea em bovinos após a aplicação simultânea dos imunógenos sintéticos anti-Babesia bovis (SBbo23290) e anti-Ripicephalus (Boophilus) microplus (SBm7462)

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Sixteen bovines belonged to Bos taurus taurus species, serumly and parasitically negatives to hemoparasites, were divided in four groups of inoculation: Control groups, Saponine group, Treatment group I and Treatment group II. The following study had the objective to evaluate and measure the cellular immune response and the protection given to bovines, through simultaneous vaccination of synthetics immunogenics SBbo 23290 in opposite sides (Treatment I) and through vaccination of the same immunogenics SBm 7462 + SB 23290 in the same dose (Treatment II). All animals were inoculated three times, through subcutaneous, with intermissions of thirty days. The challenges were done thirty and thirty-four days after third inoculation, placing 2000 grubs of Ripicephalus Boophilus miroplus, out of hemoparasites, and lately inoculating the strain of Babesia bovis UFV1 ninth passing, in the concentration of 1 x 106 Babesias/ml, respectively. The results show that, along the study, animals vaccinated in two forms related obtained an alteration of the sub population of lymphocytes present in the peripheral blood. Among the population of lymphocytes studied, a bigger increase for the sub population of cells B CD21+ was seen. Through the study of kinetic production of interleukin 4, was valued the activation of blood peripheral mononuclear cells (PBMC) cultivated after vaccination of animals and beforehand stimulated together with synthetic peptides SBbo 23290 e SBm 7462. The quantification of IL-4 production showed low levels for Treatment group I, Treatment group II and Saponine group.

ASSUNTO(S)

ripicephalus (boophilus) microplus medicina veterinaria preventiva resposta imune celular babesia bovis ripicephalus (boophilus) microplus cell immune response babesia bovis

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