Avian reticuloendotheliosis virus: characterization of genome structure by heteroduplex mapping.
AUTOR(ES)
Hu, S S
RESUMO
The genome structure of defective, oncogenic avian reticuloendotheliosis virus (REV) was studied by heteroduplex mapping between the full-length complementary DNA of the helper virus REV-T1 and the 30S REV RNA. The REV genome (5.5 kilobases) had a deletion of 3.69 kilobases in the gag-pol region, confirming the genetic defectiveness of REV. In addition, REV lacked the sequences corresponding to the env gene but contained, instead, a contiguous stretch (1.6 to 1.9 kilobases) of the specific sequences presumably related to viral oncogenicity. Unlike those of other avian acute leukemia viruses, the transformation-specific sequences of REV were not contiguous with the gag-pol deletion. Thus, REV has a genome structure similar to that of a defective mink cell focus-inducing virus or a defective murine sarcoma virus. An additional class of heteroduplex molecules containing the gag-pol deletion and two other smaller deletion loops was observed. These molecules probably represented recombinants between the oncogenic REV and its helper virus.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=171086Documentos Relacionados
- Genome of avian myelocytomatosis virus MC29: analysis by heteroduplex mapping.
- Characterization of the env gene in avian oncoviruses by heteroduplex mapping.
- Genome of Reticuloendotheliosis Virus: Characterization by Use of Cloned Proviral DNA
- Avian reticuloendotheliosis virus: characterization of the high-molecular-weight viral RNA in transforming and helper virus populations.
- Characterization of the guinea pig cytomegalovirus genome by molecular cloning and physical mapping.