Bacillus anthracis and Bacillus cereus PcrA Helicases Can Support DNA Unwinding and In Vitro Rolling-Circle Replication of Plasmid pT181 of Staphylococcus aureus
AUTOR(ES)
Anand, Syam P.
FONTE
American Society for Microbiology
RESUMO
Replication of rolling-circle replicating (RCR) plasmids in gram-positive bacteria requires the unwinding of initiator protein-nicked plasmid DNA by the PcrA helicase. In this report, we demonstrate that heterologous PcrA helicases from Bacillus anthracis and Bacillus cereus are capable of unwinding Staphylococcus aureus plasmid pT181 from the initiator-generated nick and promoting in vitro replication of the plasmid. These helicases also physically interact with the RepC initiator protein of pT181. The ability of PcrA helicases to unwind noncognate RCR plasmids may contribute to the broad-host-range replication and dissemination of RCR plasmids in gram-positive bacteria.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=374418Documentos Relacionados
- Initiation of rolling-circle replication in pT181 plasmid: initiator protein enhances cruciform extrusion at the origin.
- An 18-base-pair sequence is sufficient for termination of rolling-circle replication of plasmid pT181.
- Intermediates in plasmid pT181 DNA replication.
- Replication of single-stranded plasmid pT181 DNA in vitro.
- Plasmid pT181-linked suppressors of the Staphylococcus aureus pcrA3 chromosomal mutation.