Bacilysocin, a Novel Phospholipid Antibiotic Produced by Bacillus subtilis 168
AUTOR(ES)
Tamehiro, Norimasa
FONTE
American Society for Microbiology
RESUMO
We have found a novel phospholipid antibiotic (named bacilysocin) which accumulates within (or associates with) the cells of Bacillus subtilis 168 and determined the structure by nuclear magnetic resonance and mass spectrometry analyses. The structure of bacilysocin elucidated was 1-(12-methyltetradecanoyl)-3-phosphoglyceroglycerol. Bacilysocin demonstrated antimicrobial activity, especially against certain fungi. Production of bacilysocin commenced immediately after growth ceased and before the formation of heat-resistant spores. The production of bacilysocin was completely blocked when the ytpA gene, which encodes a protein homologous to lysophospholipase, was disrupted, but blockage of the ytpA gene did not significantly affect growth. Sporulation was also impaired, with a 10-fold reduction in heat-resistant spore titers being detected. Since the ytpA disruptant actually lacked phospholipase activity, we propose that the YtpA protein functions as an enzyme for the biosynthesis of bacilysocin.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=127064Documentos Relacionados
- Mycocerein, a novel antifungal peptide antibiotic produced by Bacillus cereus.
- Suppressor System in Bacillus subtilis 168
- Bacteriophage Interference in Bacillus subtilis 168
- Conversion of Bacillus subtilis 168 to a subtilin producer by competence transformation.
- Aspartokinase III, a new isozyme in Bacillus subtilis 168.