Bacterial Mutants Defective in Plasmid Formation: Requirement for the lon+ Allele

AUTOR(ES)

Takano, Toshiya

RESUMO

Bacterial mutants defective in plasmid formation were selected for their inability to be transduced to chloramphenicol resistance by bacteriophage PlCM. The mutants isolated are indistinguishable from a lon mutant strain. Both the lon strain and the mutants isolated here show very poor lysogenization by P1 and very low transduction to Gal+ by the plasmid formation of the λgal8-Nam7am53cI857. The lon+ gene function of the host bacteria is indispensable for plasmid formation, even though P1 and λ+ can grow normally on the lon strains and λ+ can be integrated normally in lon as well as in the lon+ bacteria. Phage mutants that can persist as plasmids in lon strains were isolated from P1CM. The function of the lon+ gene is discussed with regard to plasmid formation.

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