Bacteriocin Typing of Vibrio cholerae
AUTOR(ES)
Chakrabarty, A. N.
RESUMO
Bacteriocins of Vibrio cholerae have been demonstrated against enterobacterial and vibrio indicator organisms by conventional techniques. Abundant bacteriocin production took place on casein hydrolysate-yeast extract, tryptic soy, digest broth, proteose-peptone, and neopeptone agars. Essential factors were a citrate-phosphate buffer concentration of 0.5 to 0.7%, at pH 7.5 to 7.6, and cold shock. Thermal treatment of indicator organisms at 45 C for 12 min increased the percentage of typable strains. The bacteriocins of V. cholerae appeared to be powerful diffusible bactericidal agents. By using 8 indicator strains, 11 bacteriocin types have been recognized among 425 strains, of which 87% are typable at present.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=415895Documentos Relacionados
- Single bacteriocin typing scheme for the vibrio group of organisms.
- Development and Evaluation of a Phage Typing Scheme for Vibrio cholerae O139
- New phage typing scheme for Vibrio cholerae O1 biotype El Tor strains.
- Molecular Typing of Vibrio cholerae O1 Isolates from Thailand by Pulsed-field Gel Electrophoresis
- The Zymovars of Vibrio cholerae: Multilocus Enzyme Electrophoresis of Vibrio cholerae