Bacteriophage Mu-1-induced mutation to mutT in Escherichia coli.

AUTOR(ES)

Conrad, S E

RESUMO

Of approximately 10,000 independent phage Mu-1 lysogens, 3 had a mutator phenotype. One (mutation designated mut-49) resembled mutT1 in the frequency and types of mutations induced. mut-49 was mapped between leu and ace and was not separable from the Mu prophage. mut-49 was recessive and did not complement mutT1. mut-49, like mutT1, did not increase the reversion of the frameshift mutation lac Z (ICR48). mut-49 and mutT1 induced the same two classes of trpA78 revertants, indicating that mut-49 induced adenine-thymine leads to cytosine-guanine transversions. The results support previous work indicating that the mutational specificity of mutT is gene and not allele specific.

Documentos Relacionados

• Bacteriophage Mu-1-induced permeability mutants in Escherichia coli K-12.
• Activity of the Escherichia coli mutT mutator allele in an anaerobic environment.
• Characterization of the defect in the Escherichia coli mutT1 mutator gene.
• Functional significance of conserved residues in the phosphohydrolase module of Escherichia coli MutT protein
• Elevated mutation rate in mutT bacteria during starvation: evidence for DNA turnover?