Bacteriophage T4 RNA ligase is gene 63 product, the protein that promotes tail fiber attachment to the baseplate.

AUTOR(ES)

Snopek, T J

RESUMO

RNA ligase and tail fiber attachment activities, normally induced following bacteriophage T4 infection of Escherichia coli, are not induced when gene 63 amber mutants of T4 infect nonpermissive host cells. Both activities are induced when these mutants infect permissive hosts, or when revertants of these mutants infect nonpermissive hosts. When one of these mutants infects a host that carries supF, both activities are more than normally heat labile. RNA ligase, purified to homogeneity, promotes the tail fiber attachment reaction in vitro with a specific activity similar to that of the most highly purified preparations of gene 63 product isolated on the basis of tail fiber attachment activity. We conclude that T4 RNA ligase is gene 63 product. The RNA ligase and tail fiber attachment reactions differ in requirements and in response to some inhibitors, suggesting that the two activities of the gene 63 product may be mechanistically unrelated.

Documentos Relacionados

• Sequence and cloning of bacteriophage T4 gene 63 encoding RNA ligase and tail fibre attachment activities.
• Suppressors of mutations in the bacteriophage T4 gene coding for both RNA ligase and tail fiber attachment activities.
• Identification of the Bacteriophage T4 unf (= alc) Gene Product, a Protein Involved in the Shutoff of Host Transcription
• The asiA gene product of bacteriophage T4 is required for middle mode RNA synthesis.
• The asiA Gene Product of Bacteriophage T4 Is Required for Middle Mode RNA Synthesis