Bacteriophage T7 DNA Synthesis in Isolated DNA-Membrane Complexes
AUTOR(ES)
Center, Melvin S.
RESUMO
A DNA-membrane complex isolated from Escherichia coli infected with bacteriophage T7 contains newly synthesized T7 DNA and the T7 DNA polymerase (gene 5 product). The DNA present in the complex appears to exist as a concatemer which contains single-strand breaks and possibly internal single-stranded regions (gaps). The complex is capable of synthesizing T7 DNA by using endogenous template, and part of the DNA is made by a semiconservative mechanism. A portion of the in vitro synthesized DNA sediments in alkaline sucrose as 10-11S material. This DNA is converted to a larger-molecular-weight material after treatment with T4 polynucleotide ligase and E. coli DNA polymerase I.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=356703Documentos Relacionados
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