Ballistic Disruption of Penicillium chrysogenum Cells
AUTOR(ES)
Hamilton, Pat B.
RESUMO
The use of glass beads in a high-speed mixing device to rupture organisms was applied to molds. The use of a mixer in which the propellor shaft enters from the top into a metal mixing chamber made it possible to immerse the whole device in a salt water and ice mixture so that the temperature of the glass-bead slurry could be kept below 5 C without difficulty. Mycelia, glass beads, and buffer in a 1:2:3 (w/w) ratio gave above 95% breakage in 15 min with Penicillium chrysogenum cells and in 4 min with Rhizopus nigricans. Some of the factors influencing breakage are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1057918Documentos Relacionados
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