Bidirectional replication of plasmid R6K DNA in Escherichia coli; correspondence between origin of replication and position of single-strand break in relaxed complex.
AUTOR(ES)
Lovett, M A
RESUMO
Replicating molecules of plasmid R6K DNA have been purified as covalently closed circular DNA forms and analyzed in the electron microscopy after cleavage with the EcoRI restriction endonuclease. It has been determined that in most cases replication proceeds bidirectionally from an origin whose position is indistinguishable from the site of the single-strand break (nick) in the open circular DNA form of the relaxation complex of R6K DNA and protein. Evidence is presented for the existence of a unique replication terminus asymmetrically placed approximately 20% of genome size from the origin. The positions of the replication forks in a majority of the molecules indicate that replication proceeds sequentially from the fixed origin first in one direction to the terminus and then progresses from the origin in the other direction.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=432887Documentos Relacionados
- Amplification of single-strand DNA binding protein in Escherichia coli.
- Membrane-bound fractions of R6K plasmid DNA in Escherichia coli.
- An Escherichia coli mutant defective in single-strand binding protein is defective in DNA replication.
- Replication of the R6K plasmid during the Escherichia coli cell cycle.
- A DNA fragment from the origin of single-strand to double-strand DNA replication of bacteriophage fd.
