Binding and transcription of simian virus 40 DNA by DNA-dependent RNA polymerase from Escherichia coli.
AUTOR(ES)
Hale, P
RESUMO
Supercoiled simian virus 40 was transcribed more efficiently than nonsupercoiled DNA. The effect was increased from two- to fivefold by the addition of rifampin with triphosphates. The number and locations of polymerase binding sites with respect to Hin II-III restriction fragments were determined. The total number of binding sites was nine, as determined by UV difference spectroscopy. The locations of these binding sites were on the A, B, D, E, F, and G fragments, as determined by gel electrophoresis. The number of sites was the same for both supercoiled and relaxed or Hin II-III-digested DNA, and the point of saturation of supercoiled DNA by polymerase remained the same with increasing concentrations of rifampin from 0 to 8 microgram/ml.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=353926Documentos Relacionados
- Transcription promotes recA-independent recombination mediated by DNA-dependent RNA polymerase in Escherichia coli.
- DNA-DEPENDENT SYNTHESIS OF RNA BY Escherichia coli RNA POLYMERASE: RELEASE AND REINITIATION OF RNA CHAINS FROM DNA TEMPLATES*
- DNA-dependent single-step addition reactions catalyzed by Escherichia coli RNA polymerase.
- DNA-Dependent RNA Polymerase from Yeast Mitochondria
- Initiation by the DNA-dependent RNA polymerase.