Binding of [14C]Erythromycin to Escherichia coli Ribosomes
AUTOR(ES)
Pestka, Sidney
RESUMO
Erythromycin binding to Escherichia coli ribosomes required K+ and Mg2+. Under optimal conditions, the dissociation constant for erythromycin binding to E. coli ribosomes was found to be 1.0 × 10−8 M and 1.4 × 10−8 M at 24 C and 5 C, respectively. One molecule of [14C]erythromycin was bound to each 70S ribosome at equilibrium. Binding of erythromycin to ribosomes was rapid and reversible. The specific rate constants for the forward and reverse reactions were 1.7 × 107 liters per mol per min and 0.15 per min, respectively.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=444674Documentos Relacionados
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