Binding of Homologous Polymerized Deoxyribonucleic Acid by Streptomyces griseus
AUTOR(ES)
Germaine, G. R.
RESUMO
Germaine, G. R. (University of Minnesota, Minneapolis), and D. L. Anderson. Binding of homologous polymerized deoxyribonucleic acid by Streptomyces griseus. J. Bacteriol. 92:662–667. 1966—An irreversible P32 deoxyribonucleic acid (DNA) binding system is described for Streptomyces griseus S104. The 69% guanine plus cytosine (GC) S. griseus DNA was bound by late exponential –early stationary phase cultures. Simultaneous addition of deoxyribonuclease with the P32-DNA to maximally receptive cultures reduced the uptake by 98%. Saturation of the binding ability occurred after 20-min incubation of cells with P32-DNA. Preparative cesium chloride density gradient centrifugation of the P32-DNA revealed that 98% of the label banded in the position expected for S. griseus DNA. Sedimentation of the P32-DNA at both 23 and 37 μg/ml indicated a molecular weight of 10.8 million. Attempts to transform S. griseus S104 auxotrophs to prototrophy by use of immediate and delayed selective procedures were unsuccessful.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=276306Documentos Relacionados
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