Bioassay for the Isolation of Dictyostelium discoideum Mutants Deficient in Extracellular Accumulation of Cyclic Adenosine 3′,5′-Monophosphate
AUTOR(ES)
Sussman, Raquel R.
RESUMO
The growth response to external cyclic adenosine 3′,5′-monophosphate of a strain of Escherichia coli deleted for adenyl cyclase was utilized to screen for mutants of Dictyostelium discoideum unable to accumulate this chemical extracellularly. The threshold amount of cyclic adenosine 3′,5′-monophosphate able to induce growth of this bacterium was 3 to 4 μg/ml at 37 C and approximately 25 μg/ml at 27 C. Conditions are described that permit the detection of as low as 2 μg of this chemical at either temperature.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=246671Documentos Relacionados
- Deficient cyclic adenosine 3',5'-monophosphate control in mutants of two genes of Neurospora crassa.
- Effects of Adenosine 3′,5′-Monophosphate and Adenosine 5′-Monophosphate on Glycogen Degradation and Synthesis in Dictyostelium discoideum
- Cyclic Adenosine 3′,5′-Monophosphate in Escherichia coli
- Cyclic adenosine 3',5'-monophosphate levels and activities of adenylate cyclase and cyclic adenosine 3',5'-monophosphate phosphodiesterase in Pseudomonas and Bacteroides.
- Mutants Defective in Binding Activity for Cyclic Adenosine 3′,5′-Monophosphate in Vibrio parahaemolyticus