Biochemical and Immunological Effects of Listeria monocytogenes Hemolysin
AUTOR(ES)
Kingdon, G. Charles
RESUMO
The biochemical and immunological effects of Listeria monocytogenes hemolysin in CD-1 mice were studied. Intraperitoneal injection of 256 complete hemolytic units (CHU) caused a twofold increase in plasma β-glucuronidase levels but was not lethal. In contrast, 256 or more CHU caused 100% lethality in 4 to 5 min when administered intravenously. Intravenous administration of 50 CHU caused a 10- to 11-fold increase in plasma β-glucuronidase levels and was lethal for a variable percentage of the animals. Carbon clearance experiments showed the phagocytic index to be depressed by relatively small amounts of intravenously administered hemolysin and suggested that hemolysin may function as a leucocidal agressin during listeric infection. Increased plasma levels of ornithine carbamyltransferase after intravenous injection of hemolysin indicated hepatocellular damage. Liver carbohydrate and blood glucose determinations on fasted mice showed a reduced gluconeogenic capability in hemolysin-treated animals. Mice immunized with purified hemolysin or live vaccine were more resistant to several of the toxic parameters studied. The data indicate that hemolysin is produced during listeric infection and is antigenic, but not necessarily a protective immunogen.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=415907Documentos Relacionados
- Cardiotoxic and Lethal Effects of Listeria monocytogenes Hemolysin
- Effects of Listeria monocytogenes Hemolysin on Phagocytic Cells and Lysosomes
- Detection of listeriolysin, the thiol-dependent hemolysin in Listeria monocytogenes, Listeria ivanovii, and Listeria seeligeri.
- Chemical and Immunological Composition of Surface Structures of Listeria monocytogenes
- Transcriptional activation of the Listeria monocytogenes hemolysin gene in Bacillus subtilis.