Biochemical Studies of Bacterial Sporulation and Germination XVII. Sulfhydryl and Disulfide Levels in Dormancy and Germination

AUTOR(ES)

Setlow, Peter

RESUMO

A fourfold increase in sulfhydryl content upon germination of Bacillus megaterium spores was observed by the standard fluorescein mercuric acetate assay as reported by others. However, assay of ruptured dormant spores or the use of N-ethylmaleimide and a denaturing agent on intact spores showed a constant sulfhydryl level in dormancy and in germination. The apparent increase in sulfhydryl groups observed on germination was shown to be due to inaccessibility of most sulfhydryl groups in the dormant spore to sulfhydryl reagents. The disulfide content of dormant spores showed no change on germination, nor was any evidence found for production of low-molecular-weight sulfhydryl or disulfide compounds during germination.

Documentos Relacionados

• Biochemical Studies of Bacterial Sporulation and Germination XV. Fatty Acids in Growth, Sporulation, and Germination of Bacillus megaterium
• Biochemical Studies of Bacterial Sporulation and Germination XIV. Phospholipids in Bacillus megaterium
• Biochemical Studies of Bacterial Sporulation and Germination XXI. Temperature-Sensitive Mutants for Initiation of Germination
• Biochemical Studies of Bacterial Sporulation and Germination XIII. Adenylate Kinase of Vegetative Cells and Spores of Bacillus subtilis1
• Biochemical Studies of Bacterial Sporulation and Germination XII. A Sulfonic Acid as a Major Sulfur Compound of Bacillus subtilis Spores