Biogenesis of mitochondria: molecular mapping of the mitochondrial genome of yeast.

AUTOR(ES)

Linnane, A W

RESUMO

We have developed a new procedure for the detailed molecular mapping of any allele of the yeast (Saccharomyces cerevisiae) mitochondrial genome. The procedure employs a collection of different genetically characterized petite strains whose genomes have been physically defined by molecular hybridization. The map position of an allele is within the DNA segment common to all defined petites that can be shown by marker rescue to retain the locus. The same collection of petites can be used to locate the positions of mitochondrial rRNA and tRNA cistrons and DNA fragments produced by restriction endonucleases.

Documentos Relacionados

• Biogenesis of mitochondria: the mitochondrial gene (aap1) coding for mitochondrial ATPase subunit 8 in Saccharomyces cerevisiae.
• The initiation of DNA replication in the mitochondrial genome of yeast.
• Import of proteins into mitochondria: precursor forms of the extramitochondrially made F1-ATPase subunits in yeast.
• Biogenesis of mitochondria: a mutation in the 5'-untranslated region of yeast mitochondrial oli1 mRNA leading to impairment in translation of subunit 9 of the mitochondrial ATPase complex.
• A region of extreme instability in the mitochondrial genome of yeast.