Biological Activity of Reducing-End-Derivatized Oligogalacturonides in Tobacco Tissue Cultures1
AUTOR(ES)
Spiro, Mark D.
FONTE
American Society of Plant Physiologists
RESUMO
The biological activity of reducing-end-modified oligogalacturonides was quantified in four tobacco (Nicotiana tabacum) tissue culture bioassays. The derivatives used were oligogalacturonides with the C-1 of their reducing end (a) covalently linked to a biotin hydrazide, (b) covalently linked to tyramine, (c) chemically reduced to a primary alcohol, or (d) enzymatically oxidized to a carboxylic acid. These derivatives were tested for their ability to (a) alter morphogenesis of N. tabacum cv Samsun thin cell-layer explants, (b) elicit extracellular alkalinization by suspension-cultured cv Samsun cells, (c) elicit extracellular alkalinization by suspension-cultured N. tabacum cv Xanthi cells, and (d) elicit H2O2 accumulation in the cv Xanthi cells. In all four bioassays, each of the derivatives had reduced biological activity compared with the corresponding underivatized oligogalacturonides, demonstrating that the reducing end is a key element for the recognition of oligogalacturonides in these systems. However, the degree of reduction in biological activity depends on the tissue culture system used and on the nature of the specific reducing-end modification. These results suggest that oligogalacturonides are perceived differently in each tissue culture system.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=35035Documentos Relacionados
- “VEGETABLE DYNAMICKS” AND PLANT TISSUE CULTURES1
- Detection of Nuclease Activity in Semisolid and Broth Cultures1
- Aerosols as a Source of Widespread Mycoplasma Contamination of Tissue Cultures1
- Electron Microscope Methods for the Identification of Adenoviruses Isolated in Micro Tissue Cultures1
- Aluminum Induces a Decrease in Cytosolic Calcium Concentration in BY-2 Tobacco Cell Cultures1