Biosynthesis of Transfer RNA: In Vitro Conversion of Transfer RNA Precursors from Bombyx mori to 4S RNA by Escherichia coli Enzymes

AUTOR(ES)

Chen, G. S.

RESUMO

Evidence for the presence of rapidly labeled short-lived RNA species, presumed to be precursors to transfer RNA (tRNA), in the silk glands of Bombyx mori is presented. These precursors, which migrate between 4S and 5S markers on acrylamide gels, can be converted in vitro into molecules indistinguishable in size from tRNA. The conversion is also catalyzed by a crude enzyme fraction isolated from ribosomes of E. coli. Since a similar enzyme preparation cleaves the suppressor tRNATyr sequence of E. coli from its precursor molecule, it would appear that the precursor to E. coli tRNATyr and the precursors to silk-gland tRNAs share common structural features.

Documentos Relacionados

• Transcription in vitro of bacteriophage lambda 4S RNA: studies on termination and rho protein.
• Structure and in vitro transcription of a glycine tRNA gene from Bombyx mori.
• Methyldeficient mammalian 4S RNA: evidence for L-ethionine-induced inhibition of N6-dimethyladenosine synthesis in rat liver tRNA
• The terminal sequences of Bombyx mori 18S ribosomal RNA.
• Identification of precursor molecules to individual tRNA species from Bombyx mori.