Blinded, Externally Controlled Multicenter Evaluation of Light Microscopy and PCR for Detection of Microsporidia in Stool Specimens
AUTOR(ES)
Rinder, Heinz
FONTE
American Society for Microbiology
RESUMO
The quality parameters for the detection of microsporidia in identical sets of 50 stool samples were determined for six laboratories where technicians used light microscopy and for six laboratories where technicians used PCR. The average overall sensitivities were 67% (89% for patient samples only) for the PCR laboratories and 54% (80% for patient samples only) for the light microscopy laboratories. Specificities were 98 and 95%, respectively. Differences in results were most apparent between the individual laboratories rather than between the two major methods used.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=104932Documentos Relacionados
- A Powerful DNA Extraction Method and PCR for Detection of Microsporidia in Clinical Stool Specimens
- Identification of microsporidia in stool specimens by using PCR and restriction endonucleases.
- Evaluation of DNA Extraction and PCR Methods for Detection of Enterocytozoon bienuesi in Stool Specimens
- Relevant Criteria for Detecting Microsporidia in Stool Specimens
- Development and Evaluation of a PCR Method for Detection of the Clostridium difficile Toxin B Gene in Stool Specimens