Blotting of RNA onto ion exchange paper allowing subsequent characterization by in situ translation in addition to blot hybridization.
AUTOR(ES)
Saris, C J
RESUMO
We present the preparation of an ion exchange paper which can be used as a solid carrier in the transfer of RNA from gels. In addition to detection by blot hybridization to specific probes, transferred RNA can be characterized by cell-free translation in situ. Preparation of the paper is simple, inexpensive and reproducible. Examples of applications are shown and possible other applications are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=320821Documentos Relacionados
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