Borrelia burgdorferi Population Dynamics and Prototype Gene Expression during Infection of Immunocompetent and Immunodeficient Mice
AUTOR(ES)
Hodzic, Emir
FONTE
American Society for Microbiology
RESUMO
The population dynamics of Borrelia burgdorferi were quantified by real-time PCR targeting the flaB gene in skin (inoculation site, noninoculation site, and ear), heart (heart base and ventricle), quadriceps muscle, and the tibiotarsal joint at 1, 2, 4, 6, and 8 weeks after intradermal inoculation in C3H and C3H-scid mice. In addition, RNA transcription was assessed for several prototype genes, including flaB, ospA, ospC, dbpA, arp, vlsE, fbp, oppA-2, and p37-42. Spirochete numbers were equivalent in C3H and C3H-scid mice at 1 or 2 weeks and then declined in C3H mice, but they continued to rise and then plateaued in C3H-scid mice. Gene transcription was likewise higher in C3H-scid mice than in C3H mice, particularly at 4 or more weeks of infection. Gene transcription showed variation among tissues, with the highest levels of transcription in heart and joint tissue, which are sites of inflammation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=187352Documentos Relacionados
- Antigenic stability of Borrelia burgdorferi during chronic infections of immunocompetent mice.
- Stability of erp Loci during Borrelia burgdorferi Infection: Recombination Is Not Required for Chronic Infection of Immunocompetent Mice
- Antigenic Composition of Borrelia burgdorferi during Infection of SCID Mice
- Genetic stability of Borrelia burgdorferi recovered from chronically infected immunocompetent mice.
- Experimental Borrelia burgdorferi infection of outbred mice.