BtrI, a novel restriction endonuclease, recognises the non-palindromic sequence 5′-CACGTC(-3/-3)-3′

AUTOR(ES)

Degtyarev, Sergei Kh.

FONTE

Oxford University Press

RESUMO

The recognition sequence and cleavage positions of a new restriction endonuclease BtrI isolated from Bacillus stearothermophilus SE-U62 have been determined. BtrI belongs to a rare type IIQ of restriction endonucleases, which recognise non-palindromic nucleotide sequences and cleave DNA symmetrically within them.

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