Calcium influx through hyperpolarization-activated cation channels (Ih channels) contributes to activity-evoked neuronal secretion
AUTOR(ES)
Yu, Xiao
FONTE
National Academy of Sciences
RESUMO
The hyperpolarization-activated cation channels (Ih) play a distinct role in rhythmic activities in a variety of tissues, including neurons and cardiac cells. In the present study, we investigated whether Ca2+ can permeate through the hyperpolarization-activated pacemaker channels (HCN) expressed in HEK293 cells and Ih channels in dorsal root ganglion (DRG) neurons. Using combined measurements of whole-cell currents and fura-2 Ca2+ imaging, we found that there is a Ca2+ influx in proportion to Ih induced by hyperpolarization in HEK293 cells. The Ih channel blockers Cs+ and ZD7288 inhibit both HCN current and Ca2+ influx. Measurements of the fractional Ca2+ current showed that it constitutes 0.60 ± 0.02% of the net inward current through HCN4 at –120 mV. This fractional current is similar to that of the low Ca2+-permeable AMPA-R (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) channels in Purkinje neurons. In DRG neurons, activation of Ih for 30 s also resulted in a Ca2+ influx and an elevated action potential-induced secretion, as assayed by the increase in membrane capacitance. These results suggest a functional significance for Ih channels in modulating neuronal secretion by permitting Ca2+ influx at negative membrane potentials.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=327149Documentos Relacionados
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