Calf Crystallin Synthesis in Frog Cells: The Translation of Lens-Cell 14S RNA in Oocytes

AUTOR(ES)

Berns, A. J. M.

RESUMO

14S RNA isolated from calf-lens polyribosomes was injected into oocytes of the frog Xenopus laevis. Oocytes injected with 14S RNA and buffer contained a protein resembling the A2 chain of calf α-crystallin; oocytes injected with buffer alone contained no crystallin-like material. αA2 crystallin polypeptides were identified by various criteria: urea-gel electrophoresis under acidic and basic conditions, gel electrophoresis in sodium dodecyl sulfate, N-terminal analysis, and paper chromatography of methionine-containing tryptic peptides.

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