Calf ligated intestinal segment test to detect enterotoxigenic Escherichia coli.
AUTOR(ES)
Myers, L L
RESUMO
The ligated intestinal segment test in the young calf was utilized to establish the enterotoxigenicity of approximately 600 Escherichia coli isolates obtained from fecal specimens intestinal contents of calves with diarrheal disease. One hundred and fifty isolates were routinely tested in a single calf. There was little problem with false-positive reactions. False-negative reactions normally occurred only if the isolate was tested in the posterior 3 m of the small intestine. The ligated small intestine of the calf was distended by E. coli isolates of bovine, porcine, and human origin. Use of the calf ligated intestinal segment test is recommended to determine the enterotoxigenicity of E. coli isolates of bovine origin.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=415104Documentos Relacionados
- Pilus-mediated binding of bovine enterotoxigenic Escherichia coli to calf small intestinal mucins.
- Improvements in the passive immune hemolysis test for assaying enterotoxigenic Escherichia coli.
- Attachment of enterotoxigenic Escherichia coli to human intestinal cells.
- Lactobacillus prophylaxis for diarrhea due to enterotoxigenic Escherichia coli.
- Modified Elek test for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.
