Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence.

AUTOR(ES)

Tschudi, C

RESUMO

In Trypanosoma brucei gambiense, the Ca2+ binding protein calmodulin is encoded by three identical tandemly repeated genes. The transcripts of these genes consist of several RNA species similar in size. A 35-nucleotide spliced leader sequence is present at the 5' end of each mRNA but is not encoded by DNA contiguous to these genes. We have identified two different sites for the fusion of the leader to the mRNA. These results strongly support the idea that a novel, possibly discontinuous, transcription mechanism is used by these parasites.

Documentos Relacionados

• All subgenomic mRNAs of equine arteritis virus contain a common leader sequence.
• The 5' ends of Thogoto virus (Orthomyxoviridae) mRNAs are homogeneous in both length and sequence.
• Polygene transcripts are precursors to calmodulin mRNAs in trypanosomes.
• Many trypanosome messenger RNAs share a common 5' terminal sequence.
• 5' untranslated leader sequences of eukaryotic mRNAs encoding heat shock induced proteins.