Capped mRNA Degradation Intermediates Accumulate in the Yeast spb8-2 Mutant
AUTOR(ES)
Boeck, Ronald
FONTE
American Society for Microbiology
RESUMO
mRNA in the yeast Saccharomyces cerevisiae is primarily degraded through a pathway that is stimulated by removal of the mRNA cap structure. Here we report that a mutation in the SPB8 (YJL124c) gene, initially identified as a suppressor mutation of a poly(A)-binding protein (PAB1) gene deletion, stabilizes the mRNA cap structure. Specifically, we find that the spb8-2 mutation results in the accumulation of capped, poly(A)-deficient mRNAs. The presence of this mutation also allows for the detection of mRNA species trimmed from the 3′ end. These data show that this Sm-like protein family member is involved in the process of mRNA decapping, and they provide an example of 3′-5′ mRNA degradation intermediates in yeast.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=109091Documentos Relacionados
- Nuclear mRNA accumulation causes nucleolar fragmentation in yeast mtr2 mutant.
- Intermediates in the degradation of mRNA from the lactose operon of Escherichia coli.
- ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system
- Recognition of Yeast mRNAs as “Nonsense Containing” Leads to Both Inhibition of mRNA Translation and mRNA Degradation: Implications for the Control of mRNA Decapping
- Degradation of CYC1 mRNA in the yeast Saccharomyces cerevisiae does not require translation.