Características da formação do biofilme de Candida tropicalis eresistência a antifúngicos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

Candida tropicalis is a common non-albicans species related to nosocomial candidemia and candiduria. Most of Candida spp. infections are associated with biofilm formation on the implanted medical devices or host ephitelial cells surfaces. Sessile cells display phenotypic traits dramatically different from their free-living planktonic counterparts, such as increased resistance to animicrobial agents and host defenses. Here, we describe the characteristics of C. tropicalis biofilm formation in vitro. The XTT-reduction assay was used to monitor metabolic activities of the sessile cells during the biofilm development. Raising metabolic activity was observed until 24 h of biofilm formation and this activity showed a linear relationship with sessile cellular density. Scanning electron microscopy was used to further characterize C. tropicalis biofilms. The initial adherence of yeast cells was followed by germination, micro-colony formation, filamentation and maturation over 24 to 48h. Mature biofilms consisted of a dense network of yeast cells and filamentous forms of C. tropicalis. Increased resistance of sessile cells against fluconazole and amphotericin B was also demonstrated.

ASSUNTO(S)

candida biofilme biofilms candida

ACESSO AO ARTIGO

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