Caracterização físico-química e purificação de enzimas amilolíticas de mandioca (Manihot esculenta Crantz) cv. Zolhudinha / Characterization physical-chemistry and purification of amylolytic enzymes from cassava (Manihot esculenta Crantz) cv.Zolhudinha

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

Cassava (Manihot esculenta Crantz) is a root from a native plant, cultivated in South América, that is hightly perish on the post-harvest time. The mechanisms of the root deterioration are due to enzymatic and oxidative reactions as well as the microbiological attack. In this work were studied roots of Zolhudinha variety, EMBRAPA - IM 158, cultivated in Amazonian area, which distinguishes from others varieties by its higher amylase activity. Physicochemical properties were analyzed during the post-harvest time, the purification of α-amylase were performed to establish a possible involvement on the deteriorative process. As a non-commercial variety, the cooking time of the roots was 4.30 hours on average, with 64 % of water content and 30 % of starch. The amylase activity during the post-harvest decrease 1/3 from the original at the day 5, that matches with the reducing sugar in roots by increase of five times. The purification was achieved by two chromatography steps on DEAE-cellulose and Sephacryl S-200, providing two isozymes of α-amylase, thirteen times more purified with a recovery of 7.5 % of the protein fraction, the estimated molecular weights were between 14 and 19 kDa.

ASSUNTO(S)

manihot esculenta cassava mandioca purificação post-harvest manihot esculenta purification caracterização enzimática pós-colheita alpha-amylase enzyme characterization alfa-amilase

ACESSO AO ARTIGO

Documentos Relacionados