cDNA-AFLP analysis of gene expression differences between the flower bud and sprout-shoot apical meristem of Angelica sinensis (Oliv.) Diels
AUTOR(ES)
Guang, Yu, Jinao, Duan, Hui, Yan, Bingsheng, Song, Ziqing, He
FONTE
Genetics and Molecular Biology
DATA DE PUBLICAÇÃO
2011
RESUMO
Angelica sinensis (Oliv.) Diels (Umbelliferae) is a well-known medicinal plant mainly distributed in Gansu Province of China. Its local and global demand is significant because of its food and medicinal applications. However, the early bolting rate of Angelica sinensis (Oliv.) Diels reaches 20%-60%, which seriously affects its food and medicinal qualities. Thus, differences in gene expression between the flower bud and sprout-shoot apical meristem underwent analysis, by means of cDNA-amplified restriction fragment length polymorphism, to better understand the flowering mechanism. 64 primer sets, each of which amplified to 60 transcript-derived fragments (TDFs), were used. Among these TDFs, 26 were expressed specifically in the flower bud. After cloning and sequencing, 32 distinct sequences were obtained from these 26 TDFs, and 25 were found with homologous sequences in databases. Confirmation of differential expression of 13 sequences was obtained by semi-quantitative RT-PCR, their showing higher expression levels in flower buds. These homologous sequences encode transposable elements, pentatricopeptide repeat-containing proteins, DNA-binding transcription factors, zinc finger (B-box type) family proteins, NADP-dependent sorbitol 6-phosphate dehydrogenase (S6PDH), amongst others.
Documentos Relacionados
- GenEST, a powerful bidirectional link between cDNA sequence data and gene expression profiles generated by cDNA-AFLP
- cDNA-AFLP Reveals a Striking Overlap in Race-Specific Resistance and Wound Response Gene Expression Profiles
- cDNA-AFLP na identificação de genes relacionados a qualidade fisiológica
- cDNA-AFLP analysis of Psidium guajava L. cultivars under water stress and mechanical injury: methodological implications
- The patterns of gene expression in the tomato shoot apical meristem.