Cell cycle-dependent interleukin 1 gene expression by cultured glomerular mesangial cells.
AUTOR(ES)
Lovett, D H
RESUMO
Glomerular mesangial cell (MC)--derived IL-1 may be an important factor in the development of the hypercellularity and sclerosis characteristic of many forms of glomerulonephritis. To define the regulation of IL-1 synthesis by human MC, Northern blot analyses were performed using specific probes for monocytic IL-1 alpha and beta mRNA. Proliferating MC expressed mRNA for both IL-1 alpha and beta, whereas nonproliferating MC contained no detectable IL-1 mRNA. Synchronized MC expressed IL-1 alpha and beta mRNA within 2 h of stimulation with serum. This serum effect could be reproduced with platelet-derived growth factor and epidermal growth factor. Immune precipitations of 35S-methionine-labeled cells indicate that the mesangial IL-1 is synthesized as a 33-kD precursor protein with a pI of 7.2. Extracellular mesangial IL-1 has a pI of 7.0 and molecular weight of 17 kD, consistent with its identification as IL-1 beta. Cellular proliferation in glomerular disease may be driven in part by peptide growth factor-mediated induction of mesangial IL-1 gene expression and protein synthesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=303484Documentos Relacionados
- Cell cycle-dependent modulation of telomerase activity in tumor cells.
- Cell cycle-dependent effects on deoxyribonucleotide and DNA labeling by nucleoside precursors in mammalian cells.
- Upstream stimulatory factor regulates expression of the cell cycle-dependent cyclin B1 gene promoter.
- Cell cycle–dependent expression of CXC chemokine receptor 3 by endothelial cells mediates angiostatic activity
- Cell cycle-dependent expression of thymidylate synthase in Saccharomyces cerevisiae.
