Cell cycle-dependent variations in c-Jun and JunB phosphorylation: a role in the control of cyclin D1 expression

AUTOR(ES)

Bakiri, Latifa

FONTE

Oxford University Press

RESUMO

The transcription factor AP-1, composed of Jun and Fos proteins, is a major target of mitogen-activated signal transduction pathways. However, little is known about AP-1 function in normal cycling cells. Here we report that the quantity and the phosphorylation state of the c-Jun and JunB proteins vary at the M–G1 transition. Phosphorylation of JunB by the p34cdc2–cyclin B kinase is associated with lower JunB protein levels in mitotic and early G1 cells. In contrast, c-Jun levels remain constant while the protein undergoes N-terminal phosphorylation, increasing its transactivation potential. Since JunB represses and c-Jun activates the cyclin D1 promoter, these modifications of AP-1 activity during the M–G1 transition could provide an impetus for G1 progression by a temporal increase in cyclin D1 transcription. These findings constitute a novel example of a reciprocal connection between transcription factors and the cell cycle machinery.

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