Cell cycle variations of dinucleoside polyphosphates in synchronized cultures of mammalian cells.
AUTOR(ES)
Orfanoudakis, G
RESUMO
Zajdela hepatoma culture cells (ZHC) and mouse embryo fibroblasts (Swiss 3T3) were synchronized in G1 or S phase by serum deprivation and aphidicolin treatment, respectively, to study the variations in adenylyl nucleotide (Ap4X) pool size during the progress of the cell cycle. Only minor variations, which never exceeded a factor of 2, were observed when the Ap4X concentrations were expressed on a cellular basis. The variations were found to be strictly parallel to the ATP variations. Upon release from an aphidicolin block, the minor variations of Ap4X followed DNA synthesis and preceded cytokinesis. When the nucleotide content was compared with the amount of proteins, the faint specific cell cycle changes were almost completely damped when the cells were synchronized by serum deprivation, but remained practically unchanged in the case of aphidicolin synchronization. These results suggest that the observed variations could reflect the accumulation of some nucleotides before cell division. It is not clear yet whether the variation in Ap4X concentration is significant by itself or is simply a phenomenon resulting from changes in the ATP pool.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=365376Documentos Relacionados
- Cell cycle dependency of murine cytomegalovirus replication in synchronized 3T3 cells.
- Cell cycle phase specificity of putative cyclin-dependent kinase variants in synchronized alfalfa cells.
- Cell cycle expression of RNA duplex unwindase activity in mammalian cells.
- Repair methylation of parental DNA in synchronized cultures of Novikoff hepatoma cells.
- Diel Expression of Cell Cycle-Related Genes in Synchronized Cultures of Prochlorococcus sp. Strain PCC 9511