Cell envelope and shape of Escherichia coli: multiple mutants missing the outer membrane lipoprotein and other major outer membrane proteins.
AUTOR(ES)
Sonntag, I
RESUMO
Starting with an Escherichia coli strain missing the outer membrane lipoprotein, multiple mutants were constructed than in addition to this defect miss the outer membrane proteins II, Ia and Ib, or Ia, Ib, and II. In contrast to all single mutants or strains missing the lipoprotein and polypeptides Ia and Ib, drastic influences on the integrity of the outer membrane and cell morphology were observed in mutants without lipoprotein and protein II. Such strains exhibited spherical morphology. They required increased concentrations of electrolytes for optimal growth, and Mg2+ or Ca2+ were the most efficient. These mutants were sensitive to hydrophobic antibiotics and detergents. Electron microscopy revealed abundant blebbing of the outer membrane, and it could clearly be seen that the murein layer was no longer associated with the outer membrane.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=218658Documentos Relacionados
- Interaction between two major outer membrane proteins of Escherichia coli: the matrix protein and the lipoprotein.
- Export of protein in Escherichia coli: a novel mutation in ompC affects expression of other major outer membrane proteins.
- Major proteins of the Escherichia coli outer cell envelope membrane as bacteriophage receptors.
- The tolC locus of Escherichia coli affects the expression of three major outer membrane proteins.
- Cell shape and division in Escherichia coli: experiments with shape and division mutants.
