Cell-free Synthesis of a Specific Lipoprotein of the Escherichia coli Outer Membrane Directed by Purified Messenger RNA
AUTOR(ES)
Hirashima, Akikazu
RESUMO
A specific lipoprotein of the E. coli outer membrane has been synthesized in a cell-free system directed by purified messenger RNA. The mRNA for the lipoprotein was purified as 7S RNA about 250-fold from exponentially growing cells. Protein synthesis of the cell-free system was totally dependent upon the addition of the purified mRNA. The product of the cell-free system was identified as the specific lipoprotein by immunoprecipitation and by peptide mapping.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=434347Documentos Relacionados
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