Cell surface protein partially restores morphology, adhesiveness, and contact inhibition of movement to transformed fibroblasts.

AUTOR(ES)

Yamada, K M

RESUMO

We have isolated the major cell surface glycoprotein of chick embryo fibroblasts, CSP, and added it to a variety of transformed cells in vitro. The transformed cells become more elongated, often more flattened, and show increased adhesion to the substratum. Several transformed cell lines also align in striking parallel arrays. This alignment is characterized by a decrease in the amount of nuclear overlapping, probably indicating restoration of contact inhibition of movement. The morphological changes are antagonized by antibody to CSP. These effects of CSP are not associated with an elevation of cellular 3':5'-cyclic AMP. Moreover, the morphological reversion is not accompanied by an alteration in growth properties. Our results are consistent with a role for CSP in cell adhesion and morphology but not in growth control.

Documentos Relacionados

• Isolation and characterization of a tumor cell surface antigen from spontaneously transformed BALB/c mouse fibroblasts.
• Contact Inhibition of Movement in the Cultures of Transformed Cells
• Role of surface modulating assemblies in growth control of normal and transformed fibroblasts.
• Detection of the v-abl gene product at cell-substratum contact sites in Abelson murine leukemia virus-transformed fibroblasts.
• Morphology, Motility, and Surface Behavior of Lymphocytes Bound to Nylon Fibers